Determination of ribavirin by flow injection chemiluminescence

摘要

Objective: To establish a rapid and precise continuous flow-injection chemiluminescence(CL) method to determine ribavirin. Methods: The ribavirin could restrain strongly CL reaction of luminol in sodium hydroxide (NaOH) solution with potassium chlorate(KClO_3). The different experimental parameters affecting the CL intensity were studied carefully. Results: Under optimum conditions (The concentrations of luminol, KClO_3, and NaOH were at 0.1 mmol/L, 0.5 μmol/L, and 0.2 mol/L, respectively) , the linear range of the working curves was 0.01-7.00 μg/ml with a detection limit of 0.004 μg/ml. Conclusion: The method is simple, rapid and sensitive, and successfully applied to the determination of ribavirin in pharmaceutical preparations and biological fluids.