The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines

摘要

Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4 expression in prostate carcinoma cell lines. Methods: Small interference RNA(siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines(PC-3m and LNCaP)with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81% ± 10.20% ,56.10% ±9.32% at the 24th hour and the 48th hour, compared with 56.93% ±8.78% ,49.24% ± 11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71% ±6.68% ,58.66% ±11.56% respectively. Conclusion: The expression of CXCR4 gene can effectively be inhibited by RNAi expression vectors.