Preparation of CS/GPTMS hybrid molecularly imprinted membrane for efficient chiral resolution of phenylalanine isomers

摘要

An enantioselective chitosan (CS)/-gamma-glycidoxypropyltrimethoxysilarte (GPTMS) hybrid membrane was prepared in aqueous phase by a modified sol-gel process using CS as bulk polymer, L-phenylalanine ((L)-Phe) as imprinting molecules and GPTMS as crosslinking agent. SEM-EDX characterization reveals the dense structure of the membrane and homogeneous distribution of silica in chitosan matrix. The chiral separation ability of CS/GPTMS hybrid molecularly imprinted membranes towards the underivatized D,L-Phe aqueous mixture was evaluated by permeation and binding experiments. Significantly improved selectivity with a separation factor alpha(D/L) up to 4.5 has been achieved. (c) 2006 Elsevier B.V. All rights reserved.