Apoptosis and P53 protein expression after rat spinal cord injury

摘要

Objective: To determine the DNA damage, neuronal and glial cells apoptosis and expression of P53 protein in the rat spinal cord after compression injury. Methods: Terminal dexoynucleotidyl transferase Mediated dUPT nick end labeling (TUNEL), DNA gel electrophoresis and mimmunohistochemistry techniques Were used to detect DNA fragmentation in the injured rat spinal cord. Results: The apoptosic cells and P53 Protein presented at 4 h after spinal cord injury with a maximum presence at 24 h in the injuried T_8,9 DNA Fragmentation presented typical ladder pattern on aggarose gel at 24 h. Conclusion: There are lots of neuronal And glial cells apoptosis with DNA damage after SCI. The P53 protein may play an important role in induc- Tion of neuronal and glial cells to apoptosis.