Selection of humanized antibody against HBsAg and analysis of gene encoding its heavy chain

摘要

Objective: To select a human phage antibody against HBsAg and sequence the heavy chain and light chain gene. Methods: Human immunoglobulin combinatorical librry was generated by using phage surface display expression system, from which phage antibodies (Fab fragments) against HBsAg were screened, and their antigen binding activity and specificity were assayed by ELISA and competition inhibition ELISA.