TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine

摘要

Objective: To study the role of macrophage inflammatory protein ( MIP)-2γ in myocarditis pathogenesis in BALB/c mice. Methods: The relationship, between the progression of Coxsarckie virus B_3 (CVB_3) viral myocarditis and experimental autoimmune myocarditis and MIP-2γ mRNA expression in mouse was studied by TaqMan real-time fluorescent quantitative RT-PCR. Results: MIP-2γ mRNA expression rose on 3 to 5 d after CVB_3 infection, reached peak on 7 d, and returned to normal level until 14 d, which corresponded well with the disease course. The MIP-2γ mRNA expression level rose significantly on the day 18 d after immunization with porcine cardiac myosin which was consistent with pathological examination. Conclusion: MIP-2γ may be involved in the pathogenesis of myocarditis.