The apoptosis in various stages of infantile hemangioma

摘要

Objective: To detect the apoptosis in various stages of infantile hemangioma. Methods: Total 52 samples of infantile hemangioma (including 8 fresh samples) were included in this study. Agarose gel electrophoresis, transmission electron microscopy (TEM) and in situ TdT mediated dUTP-biotin nick end labeling (TUNED staining were used to observe the apoptosis. H-E staining was used to analyze the number of cells, the number and area of microvessels in hemangiomas. Results: The typical "ladder" occurred in the DNA electrophoresis of the hemangioma tissue in the late proferating stage. Many apoptotic cells were found in infantile hemangiomas with TEM. TUNEL staining identified that there were apoptotic cells througout the pathologic evolution of infantile hemangioma and the AI(%) was the highest in the late pro- ferating stage. There existed close relationship between the AI(%) and the total number of cells in he- mangioma. Conclusion: The decrease of cells resulted from the apoptosis may be the major cause of the spontaneous involution of infantile hemangioma.