A new PET fluorescent sensor for Zn~(2+)

摘要

A new Zn~(2+) fluorescent sensor NIDPA, with 1,8-naphthalimide as reporting group and di-2-picolylamine (DPA) as recognizing group, has been synthesized via simple steps. Based on photo-induced electron transfer (PET) mechanism, NIDPA has a five-fold fluorescent enhancement and 10nm absorption blue-shift under simulated physiological conditions corresponding to the binding of Zn~(2+). Apparent dissociation constant for Zn~(2+) (K_d) is 0.83 nM, and Ca~(2+), Mg~(2+), Fe~(2+), Ni~(2+) and Cr~(3+) have little influence on fluorescent enhancement. These results suggest that NIDPA can be used to quantitatively and selectively measure the concentration of Zn~(2+) around sub-nanomolar range.