Lipid Turnover During Inverse Flocculation in Saccharomyces cerevisiae UOFS Y-2330

摘要

In this study we uncovered that Saccharomyces cerevisiae UOFS Y-2330 does not only demonstrate inverse flocculation, but is also characterised by two different lipid turnover patterns. During Flo 1 phenotype flocculation, this yeast showed two neutral lipid accumulating stages (i.e. at 8 h and from 12 h). This is probably triggered by flocculation, which can be regarded as a survival mechanism where cells accumulate predominantly neutral lipids as a reserve energy source - a similar mechanism is probably operative when cells enter stationary growth. Contrary to Flol behaviour, this strain in NewFlo phenotype mode demonstrates only a single lipid accumulation phase i.e. when cells enter stationary growth, which coincides with increase in flocculation. In addition, an increase in phospholipids was experienced during active growth in both flocculation behaviours i.e. Flol and NewFlo probably as a result of active membrane production.