The Effect of BCG-PSN on T-cell Subsets and Cytokines in Vernal Conjunctivitis

摘要

The effects of BCG-PSN on T-cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG-PSN by allergen diagnostic instrument in vitro. The content of T-cell subsets of peripheral blood and cytokine were determined by using indirect immune fluorescence method, and IL-4 and INF-γ were quantified by ELISA. The results showed that the level of total IgE was substantially reduced (P< 0. 01) after treatment in the BCG-PSN group. Meanwhile, CD_8~+ was decreased, CD_4~+ and CD_4~+ /CD_8~+ ratio elevated with significant differences (P<0. 05) as compared with pre-treatment results. The changes in total IgE, CD_8~+, CD_4~+ and CD_4~+ /CD_8~+ ratio after treatment also presented significant differences (P<0. 05) between BCG-PSN group and routine treatment group. The level of IL-4 in serum declined (P<0. 05) after treatment in the BCG-PSN group, and INF-γ went up (P<0. 05). IL-4 and INF-γ in serum showed significant differences (P<0. 05) between two groups after treatment. It is concluded that BCG-PSN has a bi-directional immunoregulating effect. It can bring CD_4~+ and CD_8~+ into homeostasis, thereby preventing the occurrence of anaphylaxis. At the same time, BCG-PSN can restrain Th_2, decrease the synthesis of IL-4, switch the balance of Th_2/Th_2 to Th_1 side, boost up the predominance of Th_1 relatively, which is propitious to perennial stabilization and recovery of vernal conjunctivitis.