Screening of Anti-endotoxin Components from Radix Isatidis

摘要

To find a component with the strongest anti-endotoxin effect from the four components (F_(021), F_(022) , F_(023), and F_(024)) of chloroform extract (F_(02)) of Radix Isatidis , the protective effects of the five components on endotoxin-challenged mice and their effects on the production of TNFα and IL-6 by macrophages from the endotoxin-challenged mice were compared. It was found that the five components all had protective effects on endotoxin-challenged mice in terms of mortality. The mortalities of the mice challenged by 2. 42 mg/kg endotoxin were 30%, 50%, 20% , 50% and 60% after treatment with F_(02) , F_(021) , F_(022) , F_(023) , and F_(024). The 5 components all had inhibitory effects on the production of TNFα and IL-6 by macrophages from the mice. At a concentration of LPS of 50 ng/mL, the inhibitory rates of F_(02) , F_(021) , F_(022) , F_(023) , and F_(024) for the production of TNFα and IL-6 were 76. 54%, 30. 86%, 78. 60%, 36. 63%, 2. 06% and 75. 85% , 18. 45%, 77. 68%, 24. 41% , 3. 64% and at the dose of 100 ng/mL, the inhibitory rates were 49. 65% , 16. 86% , 66. 97% , 13. 39%, 7. 16% and 59. 78% , 1. 28% , 61. 86% , 2. 08% , 1. 44% , respectively. It is concluded that the component F_(022) of Radix Isatidis has the strongest anti-endotoxin effect.