Effect of Allelochemicals of Chinese-fir root extracted by supercritical CO_2 extraction on Chinese fir

摘要

Allelochemicals of Chinese-fir root was extracted by technology of supercritical CO_2 extraction under orthogonal experiment design, and it was used to analyze allelopathic activity of Chinese-fir through bioassay of seed germination. The results showed that as to the available rate of allelochemicals, the pressure and temperature of extraction were the most important factors. The allelochemicals of Chinese-fir root extracted by pure CO_2 and ethanol mixed with CO_2 have different allelopathic activities to seed germination, and the allelochemicals extracted by ethanol mixed with CO_2 had stronger inhibitory effects on seed germination than that extracted by pure CO_2.