Effect of soluble dietary fibers on lipase-catalyzed hydrolysis of tributyrin

摘要

The ability of soluble fibers to inhibit calf pregastric lipase (CPGL)-catalyzed hydrolysis of tributyrin (TBG) has been measured at pH 6.5, 37 degreesC (CPGL 0.02 mg/mL). Fibers tested were pectin, carboxymethylcellulose (CMC), carrageenan, and gum arabic. The fibers (5 g/L), solubilized in Bis-Tris buffer (50 mM, pH 6.5) at 4 degreesC for either 15 min or 24 h, were added to an emulsion containing TBG, before initiating the reaction by addition of CPGL. An immediate inhibitory effect of 8% to 19% was observed. The value of the CPGL-pectin dissociation constant, K-i, was 0.76 to 2.34 mM. Dialysis of pectin solution reduced the inhibitory effect by 14% and the concentration of galacturonic acid by 15%. [References: 31]