EXTRACTION OF LIPID FROM ABALONE (HALIOTIS DISCUS HANNAI INO) GONAD BY SUPERCRITICAL CARBON DIOXIDE AND ENZYME-ASSISTED ORGANIC SOLVENT METHODS

摘要

The objective of this study was to establish methods to extract lipids from abalone (Haliotis discus hannai Ino) gonad. Results showed that enzyme-assisted organic solvent extraction allowed recovering 60.00 ± 2.16% of abalone gonad lipid from the samples digested with neutral protease. In addition, a lipid yield of 82.56 ± 2.77% was achieved by supercritical carbon dioxide (SC-CO2) extraction. Thin-layer chromatography-flame ionization detection analysis indicated that lipid extracted by soxhlet method from H. discus hannai Ino gonad contained 89.05 ± 0.97% of triglycerides, 7.34 ± 0.80% of phospholipids, 1.46 ± 0.08% of cholesterol and 2.15 ± 0.09% of free fatty acids. The lipid extracted was divided into the unsaponifiable fraction (sterol) and the saponifiable fraction, and analyzed by gas chromatography mass spectrometry. Results demonstrated that the compositions of sterol and fatty acid were different for samples extracted by different methods, indicating that the extraction method could influence the composition of the lipids.