Simultaneous determination of cholesterol, stigmasterol, and β-sitosterol contents in milk and dairy products

摘要

Since milk and dairy products are largely consumed food in the world, authenticationis very important to control their real quality. Thus, the aim of this study was todevelop and validate a rapid and reliable HPLC-UV-DADmethod for the simultaneousdetermination of cholesterol, stigmasterol, and β-sitosterolcontents in variousdairy matrices (milk, cream, cheese, and butter). The validation showed great accuracyand precision of the developed method with the limit of detection of 2.32, 3.29,and 0.99 μg/ml for cholesterol, stigmasterol, and β-sitosterol,respectively. Except forbutter samples, the presence of phytosterols was not detected in other dairy samplesat given analytical conditions. It can be concluded that the proposed HPLC methodis suitable for simultaneous determination of cholesterol and phytosterol contents inmilk and dairy products and it can serve as an authentication tool in food fat analysis.