IN VITRO ON MICROBIALLY TREATED AND UNTREATED SAGO FACTORY EFFLUENT ON GROWTH OF VIGNA MUNGO AND V. RADIATA

摘要

Physico chemical parameters were estimated in sago factory effluents collected from Salem, Tamil Nadu and effluent was diluted in varying concentrations (5.10,25,50,75&100%)and used for the seeds (Vigna mungo & V. radiata). Starch degraders were isolated from the' same effluent (Alcaligenes, Bacillus & Pseudomonas sp). Combination of degrading organisms including Bacillus and Pseudomonas and Alcaligenes were inoculated into the effluents. The untreated effluent used showed seed germination of V.mungo (80%) and V.radiata (88%), and a poor response against control seeds. The treated effluent showed better response in seed germination of V.Mungo (84%) than control seeds. Effluent treated / untreated plants did not influence germination in V radiata. When shoot length and root length of V. mungo were measured at 10% concentration of treated effluent, there is a recovery of 0.9 cm of shoot length and 6 cm of root length over control. As for V. radiata, there is loss of only 1.2 cm for shoot length over control at 10% concentration treated effluent, while root length was not affected by the effluent at all. The effluent influenced both germination and shoot / root lengths to some extent in V. mungo, whereas it has little effect on V. radiata. However above 10% of effluent, bacterial treatment could not recover the growth of shoot / root in both the plants tested.