EFFECT OF 2, 4-D HERBICIDE ON HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF MALE WISTAR RATS

摘要

The effect of herbicide 2.4-D (dimethyl-amine salt DMA) was studied on haematological and biochemical parameters of male Wistar rats. To observe the toxic effects of 2,4-D, rats were given oral dose of 25 % of LD_(50) of the 2, 4-D (12 mg/kg/body weight) dissolved in water for 120 days. Haematological analysis showed significant increase in total leucocyte count (TLC) (7683.33±511.53 thousand/cu mm) particularly in Eosinophill with slight decrease in Monocyte (1.5±0.547%). while no alterations were found in RBC count and haemoglohin (%). Biochemical analysis of blood exhibited significantly increased glucose (322.33±51.067 mg/dl) and sodium (143.66± 1.966 mEq/l) level. It has been also observed that 2,4-D induced testis atrophy, swelling on intestine and kidney. Symptomatic behavioural changes were also observed. The use of 2, 4-D herbicides in agricultural field induces health problems due to environmental persistence and increase in concentration during the passage through food chain