Partially purified paeoniflorin exerts protective effects on UV-induced DNA damage and reduces facial wrinkles in human skin

摘要

Partially purified paeoniflorin(PF),a new cosmetic ingredient from roots of Paeoniae lactiflora,has been developed.Its paeoniflorin content is about 64%,far higher than that of conventional,cosmetic-grade peony root extracts(approx 10%).In this report,we studied the effects of PF on UV-induced DNA damage in both cultured human keratinocytes and hairless mouse skin.We also investigated the anti-wrinkle effects of PF-containing cosmetic preparations on human skin.From the in vitro and in vivo comet assay,it was revealed that PF protected cells from DNA damage induced by ultraviolet B(UVB)irradiation in both cultured normal human keratinocytes(19.4% decrease at 0.001%)and hairless mouse skin keratinocytes(41% decrease at 0.01%).An eight-week clinical trial using 0.5% PF-containing formulation with 20 volunteers resulted in a statistically significant reduction in facial wrinkles(p < 0.05).These results suggest that the partially purified paeoniflorin has potent anti-aging and anti-wrinkle activities and should be a useful ingredient for these purposes.