A comparative study of digoxigenin, 2, 4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation

摘要

Conclusions-3' and 5' solid-phase label- ling with triple DNP groups produced the best labelling for non-isotopic ISH using deoxyoligonucleotide cocktails. Aim-To determine the optimum form of Labelling and the most efficient reporter Molecule for non-radioisotopic in situ Hybridisation (ISH). Methods-Nine deoxyoligonucleotides Complementary to histone mRNA were Synthesised and labelled either enzymati- Cally or during solid-phase synthesis with The reporter molecules digoxigenin, 2, 4,- Dinitrophenyl (DNP), or alkaline phos- Phatase.