A novel quantitative immunoassay system for p~53 using antibodies selected for optimum designation of p~53 status

摘要

Conclusion-The p~53 ELISA system is a non-competitive double monoclonal anti- body sandwich method, using CO7 and 1801 which have been shown to be highly specific for p~53 protein by immunohisto- chemistry and western blot analysis. The lower threshold of the assay is 0.1 ng/ml analyte in an enriched recombinant p~53 preparation. As p~53 is now regarded as a protein associated with prognosis in breast and other cancers, the assay may have clinical applications. Aim-To develop a highly sensitive and Specific enzyme linked immunosorbent Assay (ELISA) system for analysis of p~53 Protein in cancer lysates.