Asymmetric conduction in biological nanopores created by high-intensity, nanosecond pulsing: Inference on internal charge lining the membrane based on a model study

摘要

Nanosecond, high-intensity electric pulses have been reported to open rectifying pores in biological cell membranes. The present goal is to qualitatively understand and analyze the experimental current-voltage (Ⅰ-Ⅴ) data. Here, nanopore transport is probed using a numerical method and on the basis of an analytical model. Our results show that geometric asymmetry in the nanopore would not yield asymmetry in the Ⅰ-Ⅴ characteristics. However, positive surface charge lining the pore could produce characteristics that compare well with data from patch-clamp measurements, and a value of ～0.02 C/m~2 is predicted from the numerical calculations.