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首页> 外文期刊>Japanese journal of applied physics >Ultrasound-Mediated Gene Transfection In vitro: Enhanced Efficiency by Complexation of Plasmid DNA
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Ultrasound-Mediated Gene Transfection In vitro: Enhanced Efficiency by Complexation of Plasmid DNA

机译：体外超声介导的基因转染：通过质粒DNA的复合提高效率

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摘要

Ultrasound-mediated gene transfection in the presence of microbubbles is a recently developed promising nonviral gene delivery method. The main obstacle towards its clinical application is its low transfection efficiency. In this work, we investigate the effect of the complexation of plasmid DNA (pONA) into polyplex micelles on the transfection efficiency. Complexation changes the structure of pDNA and results in the condensation in size and enhanced stability. Both naked and complexed pDNAs were transfected into cultured cells using ultrasound in the presence of microbubbles. The transfection rate using complexed pDNA is considerably enhanced (from ～0.92 to ～1.67%, by ～82%) compared with the rate using naked pDNA. Our method provides an alternative for the improvement of the transfection efficiency of the ultrasound-mediated method.
机译：存在微泡的超声介导的基因转染是最近开发的有希望的非病毒基因递送方法。其临床应用的主要障碍是其低转染效率。在这项工作中，我们调查了质粒DNA（pONA）络合成多聚体胶束对转染效率的影响。络合会改变pDNA的结构，并导致大小缩合和稳定性增强。在存在微泡的情况下，使用超声将裸露的pDNA和复合的pDNA都转染到培养的细胞中。与使用裸pDNA的转染率相比，使用复合pDNA的转染率显着提高（从〜0.92〜〜1.67％，〜82％）。我们的方法为提高超声介导方法的转染效率提供了替代方法。

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  • 来源
    《Japanese journal of applied physics 》 |2012年第7issue2期| p.07GF29.1-07GF29.5| 共5页
  • 作者

    Yiwei Zhang; Rie Tachibana; Akio Okamoto; Takashi Azuma; Akira Sasaki; Kiyoshi Yoshinaka; Kensuke Osada; Kazunori Kataoka; Shu Takagi; Yoichiro Matsumoto;

  • 作者单位

    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    The National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8564, Japan;

    Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine,The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo, Tokyo 113-8656, Japan;

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