Sensor chip design for increasing surface-plasmon-assisted fluorescence enhancement of the V-trench biosensor

摘要

A sensor chip design for the V-trench biosensor, which is an instrument for highly sensitive fluorescence assay, was investigated to increase its sensitivity. A simulation based on the transfer matrix method revealed that the vertex angle and electric field enhancements of the V-trench biosensor chip are increased by employing a high-refractive-index material for the chip. It was proved that a chip made of high-refractive-index glass for press molding exhibited a 1.4-fold larger electric field enhancement than that made of polystyrene. Influenza virus detection was also demonstrated using glass chips, and a detection limit of 10(4) pfu/mL was obtained with a sample volume of 15 mu L. (C) 2016 The Japan Society of Applied Physics