Cell descent caused by boundary curvature of a high topographical structure for a device that changes cell density

摘要

Noninvasive techniques of controlling cell migration on substrates are widely useful for tissue engineering. However, the cell migration controls of previous studies were not enough for collecting cells locally. To solve this problem, in this work, we report the C2C12 mouse myoblast cell migration difference (descend or be repelled) by changing the curvature of the boundary of a topographical structure when the cells move from a flat surface to the boundary. 69% of the cells coming across a round boundary-the curvature radius of which was 50 mu m-descended into the hole. In contrast, no cells descended into a groove with a linear boundary. Moreover, we demonstrated the cell spatial density change from the difference at the boundary. This finding will provide a new device that will enable us to manipulate spatial cell density noninvasively for tissue engineering. (C) 2017 The Japan Society of Applied Physics