Speed quantification and tracking of moving objects in adaptive optics scanning laser ophthalmoscopy

摘要

Microscopic features of the human retina can be resolved noninvasively using an adaptive opticsnscanning laser ophthalmoscope (AOSLO). We describe an improved method to track and quantify the speed ofnmoving objects in AOSLO videos, which is necessary for characterizing the hemodynamics of retinal capillaries.nDuring video acquisition, the objects of interest are in constant motion relative to the background tissue (objectnmotion). The background tissue is in constant motion relative to the AOSLO, due to continuous eye motion duringnvideo recordings (eye motion). The location at which AOSLO acquires data is also in continuous motion, sincenthe imaging source is swept in a raster scan across the retina (raster scanning). We show that it is important tontake into consideration the combination of object motion, eye motion, and raster scanning for accurate quantifi-ncation of object speeds. The proposed methods performed well on both experimental AOSLO videos as well asnsynthetic videos generated by a virtual AOSLO. These methods improve the accuracy of methods to investigatenhemodynamics using AOSLO imaging.