Simplified approach for quantitative digital holographic phase contrast imaging of living cells

摘要

Many interferometry-based quantitative phase contrast imaging techniques require a separately generatedncoherent reference wave. This results in a low phase stability and the demand for a precise adjustment of thenintensity ratio between object and reference wave. To overcome these problems, the performance of a Michelsonninterferometer approach for digital holographic microscopy was analyzed that avoids a separately generated referencenwave by superposition of different image areas. It is shown that this simplified arrangement yields improvednphase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstratenthe capability of the method for reliable quantitative phase contrast imaging.