Effects of Systemic Octreotide, Local Mytomycine-C and Local Corticosteroids on Wound-Healing Reaction after Glaucoma Surgery

摘要

Purpose: To determine and compare the effectiveness of octreotide,mitomycine-C and corticosteroids on wound-healing reaction after glaucomasurgery. Methods: A full thickness scleral trephination was carriedout by the same surgeon on tour groups of six rabbits. A sponge soaked inmytomicine-C was applied subconjunctivally in group 1 before trephination.Group 2 received corticosteroid drops tid topically for 14 days. Group 3 received subcutaneous octreotide injections tid for 14 days. The controlgroup (group 4) was not given any drug that may interfere with wound healing.All groups received gentamycine drops tid for seven days. The rabbits wereSacrificed on the fourteenth day and the trephination area with overlyingconjunctiva was excised. The samples were prefixed with glutaraldehyde,dehydrated and embedded in Araldite Cy 212. Ten semithin sections stainedwith toluidin blue were analysed for each group. Fibroblast and macrophagecounts were performed on the surgical site and subconjunctival area.Results: Intensive fibroblastic activity, increased number of vessels andactive macrophages were observed only in group 4. The fibroblast and macrophagedensities in this group were significantly higher than the other three groups inwhich wound healing was modulated (p < 0.001). Mean number of fibroblasts ingroup 1 was also significantly less than the ones of groups 2 and 3(p < 0.01). Macrophage densities were similar in groups 1, 2 and 3. No statistical significance was found between groups 2 and 3 by means offibroblast and macrophage densities. Conclusion: Octreotide reducedwound-healing reaction in a similar fashion to corticosteroids ormitomycine-C. These initial results seem promising.