Effect of microwave irradiation pretreatment of cow dung compost on bio-hydrogen process from corn stalk by dark fermentation

摘要

The bio-hydrogen production potential from corn stalk was significantly affected by microwave irradiation pretreatment of cow dung compost in batch tests. The maximum hydrogen yield of 144.3 ml/g-corn stalk and hydrogen production rate of 3.6 ml/g-corn stalk h~(-1) were observed using the pretreated compost by microwave radiation of 1.5 min at fixed Na_2CO_3 dosage of 800 mg/1, Fe dosage of 400 mg/1, substrate concentration of 20 g/1, which increased about 99.6% and 85.2% compared with that of the control. The effects of microwave irradiation on microbial characteristics were further discussed by Atomic Force Microscope (AFM), determination of protein content and PCR-DGGE. The four dominant hydrogen-producing strains had been isolated and confirmed to be Bacillus amylolique/a-ciens, Bacillus licheni/ormis, Bacillus subtilis and Enterococcus faecium, respectively. The diversity and symbiosis relations of the mixed bacteria were also observed in fermentation hydrogen production process.