An efficient method for the large scale isolation of naringin from pomelo (Citrus grandis) peel

摘要

An efficient and simple method was developed to isolate high-purity naringin. a bitter flavoured (to humans) flavanone glycoside with various pharmacological activities, from pomelo (Citrus grandis Osbeck) peel in high yield. The process, which involves a simple methanol extraction followed by crystallisation in water with the addition of 14-15% (v/v) dichloromethane, gave four times higher yield compared to the same method with no dichloromethane addition and five times higher yield compared to the conventional direct hot water extraction. Through this process, albedo peels from four pomelo cultivars, Khao Taeng-gwa, Khao Yai, Khao Nam Pheung and Tong Dee, gave 24, 22, 20 and 16 mg of naringin g~(-1) of peel dry weight, respectively. High Performance Liquid Chromatography and Nuclear Magnetic Resonance analysis of the obtained naringin indicated >98% purity. Large scale isolation was also demonstrated.