Diagnosis of tuberculosis molecular markers based on the identification of the immune activity of mycobacterium tuberculosis

摘要

In this paper, the highly immunogenic genes Ag85b, Rv3872, CFP10, and ESAT6 of Mycobacterium tuberculosis were expressed tandemly and integrated into the carrier pTIC6a, and the recombinant plasmid was transformed into BCG and the Mycobacterium smegmatis (M.S.) by means of electric transformation to obtain the recombinant BCG (rBCG) and recombinant Mycobacterium smegmatis (rM.S.). The recombinant bacteria mice. The mice's serum antibody, splenocyte proliferation ability, and level of secretion of interferon-gamma and interleukin-4 in the splenocyte-stimulating supernatant 10 weeks after immunization were detected. The results showed that the serum antibodies and proliferative ability of spleen cells of mice in rBCG group were significantly higher than that of the ones in BCG group since the fourth week after immunization, and the IFN-gamma secretion in rBCG group was higher than that in BCG group 10 weeks after immunization, and the difference was significant (P = 0.022). The above results showed that rBCG could induce higher cellular and humoral immune response at the same time and had a more promising application. Although rM.S. vaccine can stimulate the immune response of the organism to some extent, the intensity and persistence of the immune response are inferior to that of BCG and rBCG.