Design and development of a simple laboratory model to detect 15N enrichment in cyanobacterial biomass and extra cellular ammonia using 15N gas

摘要

A laboratory scale working model that could detect the 15N enrichment in cyanobacterial biomass and extracellular ammonia, using 15N gas under in vitro conditions was designed and fabricated. Using the model, 15N enrichment of 0.48% atom excess was detected in the cyanobacterial biomass on the 30 d after inoculation. The 15N enrichment increased linearly in the extracellular ammoniacal fraction from the 20 d onward. The model would prove to be a useful tool to quantify the extent of 15N enrichment under in vitro conditions using 15N gas.