Biosynthesis and characterization of silver nanoparticles from Sargassum wightii and its antibacterial activity against multi-resistant human pathogens

摘要

January 2019; April In association with human health there is a growing need to develop nanomaterials using biological approach which must be ecofriendly, non-toxic, and cheaper. Therefore, the present research analyses were framed to synthesis and characterize silver nanoparticles from the extracts of seaweed S. wightii. The synthesized silver nanoparticles were subjected to UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy, High Pressure Liquid Chromatography, X-Ray Diffraction and antibacterial activity of agar well diffusion method. The formation of Ag-NPs was confirmed through the presence of an intense absorption peak at 420 nm using UV-Visible spectroscopy. The HPLC profile showed the presence of six secondary metabolites. The FT-IR spectra of biosynthesized AgNp of present biomolecules are amino acids, esters, polysaccharides, phenols, alkanes, chlorophyll, protein amide-I band, protein amide II band, carboxylic group, glycosidic linkage, carbohydrate, and proteins etc. The morphology of biosynthesized AgNps as reported to be spherical in shape was documented by SEM. The nanoparticles are crystalline in structure was confirmed by XRD. The results of antibacterial activity against the gram positive and gram-negative bacteria of human pathogens confirmed and elucidate that the seaweed synthesized nanoparticles could play a profound and reliable role in nano based medicine therapy.