Production and characterization of thermostable gelatinase from Bacillus globisporus isolated from Unkeshwar hot spring of Maharashtra

摘要

An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded district in Maharashtra. It was identified as Bacillus globisporus (APP11) by observing its morphological characters, microscopic features, biochemical pattern and physiological characterization. It showed luxuriant growth and thermostable gelatinase production at 50 degrees C temperature. Effect of various agro-industrial residues on gelatinase production was determined. Maximum gelatinase production (675 +/- 6.16 U/mg) from APP11 isolate was recorded after 24 h of time period using fish scale powder in the medium. Partially purified APP11 gelatinase was remarkably active at pH range 6 to 9 and temperature range 35 to 65 degrees C. Km and Vmax values of partially purified APP11 gelatinase were 3.0 mg/mL and 1000 U/mg respectively. The APP11 originated gelatinase showed remarkable activity in presence of selected metal ions.