Bacterial Tolerence and Utilization of Azo Dye as Carbon Source or Nitrogen Source or Both Carbon and Nitrogen as Azo Dye Degradation by Selective Bacterial Isolates

摘要

Microorganisms that have the ability to decolourize dyes have been reported by several researchers.Although several bacterial strains are known to decolourize several dyes, which are difficult for practical application because of their difficulty for survival in and adaptability to wastewater. But application of microorganisms has prooved to be more effective in successful degradation of the dyes.Orange G is present under the class of monoazo dye and it is valuable acid dye used in many straining methods including papani colour's OG stain. All the strains were found to resist, sustain upto 75 ppm above which only P. flurocenes, B.subtilis, Pantoea aqqlomerans,P.pyogenes,E.dissolvens and E. coli were able to tolerate. All of them were found to utilize orange G as a sole carbon or/and nitrogen source with distinctive decolouration while the pH optima of these strains ranged from 8 to 10. Their temperture optima were 37℃ except one E.coli 44℃. In accordance with the abundant occurrence in the soil ecosystem P. flurocenes were found to decolourize orange G more effectively that other strains (assay was carried out by both plate and spectro method).