Conducting Polymer Based Nucleic Acid Sensor For Environment Monitoring

摘要

Nucleic acid sensor based on polyaniline has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (CIC_4~-) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) chemistry. These dsCT-DNA-PANI/ITO and PANI/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, and Fourier-transform-infra-red (FTIR) measurements. This disposable dsCT-DNA-PANI/ITO bioelectrode is stable for about four months, can be used to detect arsenic trioxide (0.1 ppm) in 30 s.