RADIOPROTECTIVE EFFECT OF GINKGO BILOBA AGAINST GAMMA RADIATION-INDUCED CHROMOSOMAL ABERRATIONS IN HUMAN LYMPHOCYTES IN VITRO

摘要

The aim of the present study was to investigate the ra-dioprotective effect of Ginkgo biloba (GB) leaf extract on gamma (γ) radiation-induced chromosomal aberrations (CAs) in human lymphocytes. CAs were classified into 6 terms (break, dicentric, acentric, fragment, gap and ring). The mitotic index (MI) and the number of aberrant meta-phases (AMN) were also calculated.rnThe 6 groups of the lymphocyte culture were prepared. Group I did not receive any γ-radiation or GB, group II received only a 150 μg/ml dose of GB, group III received γ-radiation at a dose of 3 Gy, whereas groups IV, V and VI received 25, 75 and 150 μg/ml doses of GB before radiation.rnThe results indicated that all GB extract-supplemented lymphocytes had a lower frequency of CAs than those in group III. It was seen that the 150 μg/ml dose of GB was much more protective than the other doses for CAs. Besides, MI increased and AMN decreased in a dose-dependent manner. In vitro results showed that GB is a potent protector against CAs damages induced by γ-radiation, and its radioprotective role is dose-dependent.