LIGNINASE SYNTHESIS AND DECOLORIZATION OF DRIMARINE BLUE K2RL BY PLEUROTUS OSTREATUS IBL-02 IN CARBON AND NITROGEN SUFFICIENT SHAKE FLASK MEDIUM

摘要

An indigenously isolated basidiomycete Pleurotus ostreatus IBL-02 was used for decolorization of reactive textile dye Drimarene Blue K2RL (C.I. Reactive Blue 209). In initial time course trial P. ostreatus caused 69.2% decolorization of 0.01% Drimarene Blue K2RL synthetic solution after 5 days using Kirk's basal nutrient medium in shake flask cultures. Effect of varying pH, temperature, carbon and nitrogen sources and initial dye concentration on dye decolorization was investigated. By optimization of decolorization parameters, % decolorization of Drimarene Blue K2RL solution could be enhanced to 97.86 % in 3 days at pH 3 and 30 ℃ by the addition of 1% glucose (carbon source) and 1% ammonium sulphate (nitrogen source). The optimally de-colorized culture filtrates were analyzed for lignolytic enzymes. Ligninase assays showed that the fungus synthesized all ligninases. Lacasse (460 U/mL) was the major enzyme involved in dye degradation, followed by manganese peroxidase (443 U/mL) and lignin peroxidase (157 U/mL).