HIGH ENRICHMENT OF PRION PROTEIN-PrP~(Sc)-FROM BSE INFECTED BRAIN HOMOGENATES BY ADSORPTIVE BUBBLE SEPARATION (ABS)

摘要

A new enrichment method for prion protein (PrP~(Sc)) from cattle brain on the basis of adsorptive bubble separa-tion(ABS) using sodium dodecylsulfate (SDS) as surface active component was developed. The obtained results demonstrate, that prion protein (PrP~(Sc)) could be enriched 140 fold in the foam phase and determinate therefore very sensitive with officially accreditated rapid assay tests. By this way very weak contaminated brains, which lead to negative results in the routine analysis, could be positively tested.