IDENTIFICATION OF NOVEL QUININE METABOLITE FROM MARINE ACTINOMYCETES WITH ANTIFUNGAL AND ANTICANCER BIO-PROSPECTIVE

摘要

In the present study, Streptomyces violaceorubidus, Streptomyces variabilis, Streptomyces gibsonii and Streptomyces althioticus capable of producing antifungal and anticancer metabolite were isolated and identified based on 16S rRNA gene sequencing from different marine samples. S. violaceorubidus SCA-1 showed strong antifungal activity, were mass cultivated and the pure compound was characterized based on C-13 nuclear magnetic resonance (NMR), H-1 NMR, infrared, and mass spectral data. The extracellular fermentation broth of the strain SCA-1 significantly inhibited the growth of filamentous fungi. Based on the spectral data, the extracellular compounds were identified as benzo quinone in nature. The minimum inhibitory concentration of the compound against Aspergillus oryzae was 12.5 mu g mL(-1) and that against A. fumigates and A. niger (25 mu g mL(-1)), A. clavatus (50 mu g mL(-1)), and Fusarium oxysporum (100 mu g mL(-1)), respectively. IC50 values were calculated as 12.5 and 3.12 mu g mL(-1) for Vero and Mcf7 cell lines, respectively. The results indicated that the compound was comparatively nontoxic to normal cells. These results confirmed that marine actinomycetes are a potential source of anti-fungal compounds, and that S. violaceorubidus SCA-1 could be used to control the fungal pathogens.