The antifungal compounds were purified from fermentation liquid of marine actinomycetes BM-2 via silica gel column chromatography, Sephadex LH-20, thin layer chromatography and HPLC. Four compounds, Aa, Ab, Ba and Ca showing considerable antifungal activity to Fusarium graminearum were separated. Among the 4 compounds, Ca showing the highest in concentration was identified as dibutyl phthalate with ESI-MS, 1H NMR, 13C NMR spectra analysis.%从海洋放线菌菌株 BM-1发酵液中分离抗植物病原真菌的活性物质。以对禾谷镰刀菌 Fusarium graminearum的生物活性为跟踪,采用硅胶柱层析、Sephadex LH-20柱层析、薄层层析和HPLC等技术,海洋放线菌菌株BM-1发酵液中含有4种对禾谷镰刀菌具有抑菌作用的活性物质,对其进行分离纯化,得到了4种具有强抗菌作用的组分Aa、Ab、Ba、Ca。采用ESI-MS、1H NMR、13C NMR等波谱分析技术,对分离量较大、活性较强的活性组分Ca进行结构鉴定,鉴定Ca为邻苯二甲酸二丁酯(DBP)。
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