Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes

摘要

The effect of dietary flavonoids (flavone, and flavonol derivatives) on the differentiation of 3T3-L1 adipocytes was investigated. Glycerol-3-phospahte dehydrogenase (GPDH) activity, which is a hallmark of 3T3-L1 differentiation, was measured. The GPDH activity in the cells treated with quercetin, kaemp- ferol, and isorhamnetin was significantly lower than that in the control cells. A low lipid accumulation in cells stained with Oil-Red O was observed following treatment of these flavonols. The anti-differentiation action of quercetin was more potent than that of its derivatives. Quercetin directly inhibited the activity of GPDH extracted from mature 3T3- L1 adipocytes. The inhibitory action of quercetin on the differentiation of 3T3-L1 cells may involve the direct inhibi- tion of GPDH, which is a key enzyme lipid synthesis.