Simple and Rapid Analysis of Trenbolone and Zeranol Residues in Cattle Muscle and Liver by Stack-Cartridge Solid-Phase Extraction and HPLC Using On-line Clean-up with EC and UV Detection

摘要

A simple and rapid analytical method was established for detection of anabolic agents, zeranol and α-trenbolone and β-trenbolone residues in cattle muscle and liver. This method consisted of aqueous acetonitrile extraction and successive solid-phase extrac- tion with a tandem connected octadecyl (C18)- aminopropyl (NH2) cartridge, followed by on-line clean-up using multidimensional HPLC with EC and UV detection.