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Comparative study of the protein fraction of goat milk from the Indigenous Greek breed and from international breeds

机译:希腊土著品种和国际品种山羊奶蛋白质成分的比较研究

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Individual goat milk samples, taken from animals of the Indigenous Greek breed and from the international breeds Saanen and Alpine, were studied by RP-HPLC regarding the qualitative and quantitative characteristics of their proteins. Thirty-two samples from the Indigenous Greek breed and 17 from the international breeds were characterised further by RP-HPLC/ESI-MS. The mean total protein content of the milk samples from the Greek breed was higher 38.8 g/l, compared to 31.9 g/l of those from international breeds, due to the great difference between their mean αs1-Cn contents (6.90 and 3.02 g/l, respectively). In the milk samples of the Greek breed, the strong asl-Cn variants B3, B4 and As/Bl predominated, whereas in the milk samples from international breeds the medium variant E and the defectives F and null predominated. Variant A of αs2-Cn followed by variant C and the κ-Cn D (former B) were the most abundant in both groups. αs2-Cn F and the rare κ-Cn variant C/B were observed in the milk samples from the Greek breed. The β-caseins A and C were present in both groups of samples. Finally, the level of phosphorylation of the different genotypes is showed.
机译:通过RP-HPLC研究了从希腊土著品种的动物以及国际品种Saanen和Alpine提取的单个山羊奶样品的蛋白质定性和定量特征。 RP-HPLC / ESI-MS进一步表征了来自希腊土著品种的32个样品和来自国际品种的17个样品。希腊品种的牛奶样品的平均总蛋白质含量比国际品种的牛奶的平均总蛋白含量高38.8克/升,而国际品种的牛奶的平均总蛋白含量为31.9克/升,这是因为它们的平均αs1-Cn含量之间存在很大差异(6.90和3.02克/升)。 l分别)。在希腊品种的牛奶样品中,主要的asl-Cn变异体B3,B4和As / B1占主导地位,而在国际品种的牛奶样品中,中等变异体E和缺陷品F和无效氨基酸占主导地位。两组中,αs2-Cn的变体A,其次是变体C和κ-CnD(前者B)含量最高。在希腊品种的牛奶样品中观察到αs2-CnF和稀有的κ-Cn变异C / B。两组样品中均存在β-酪蛋白A和C。最后,显示了不同基因型的磷酸化水平。

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