In vivo and in vitro antioxidant activity and α-glucosidase, α-amylase inhibitory effects of flavonoids from Cichorium glandulosum seeds

摘要

The aim of this study was to investigate the antioxidant, anti-glucosidase and anti-amylase activities of total flavonoids (TFs) from Cichorium glandulosum seeds, and to analyse its chemical composition by HPLC-ESI/MS. In vitro study, radical scavenging IC_(50) values of TFs were 7.33 ±0.093, 9.24 ±0.100, 154.33 ±11.38 and 256.7 ±4.86 ug/ml for DPPH, ABTS, hydroxyl radicals, and superoxide anion, respectively. In the 8-64 mg/ml range, α-glucosidase and α-amylase were inhibited by TFs to a certain extent. In vivo, the treatment groups with TFs (100,200,400 mg/kg) showed a significant decrease in the malondi-aldehyde level, the superoxide dismutase and glutathione levels were restored to almost normal levels, and the catalase and glutathione peroxidase levels significantly increased compared to the CC14-intoxicated group in rats. The present study suggests that C glandulosum seeds should be given special attention because of their antioxidant and anti-glucosidase, anti-amylase activity.%Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China,College of Pharmacy, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, PR China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Institute of TCM & Natural Products School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China;