Preparative separation of triterpene alcohol ferulates from rice bran oil using a high performance counter-current chromatography

摘要

A novel method for the separation of two major triterpene alcohol ferulates from rice bran oil (RBO) was developed using a high performance counter-current chromatography (HPCCC). A two-phase solvent system of n-hexane-acetonitrile (1:1, v/v) was applied to purify cycloartenyl ferulate (CAF) and 24-methy-lene cycloartanyl ferulate (24-mCAF) from RBO. The yields were 20.50 ± 2.60 mg CAF and 12.62 ± 1.15 mg 24-mCAF from 390 mg RBO through a two-step separation procedure. The purities of the two compounds were 97.97 ± 0.90% and 95.50 ± 0.75%, respectively, as determined by high performance liquid chromatography (HPLC). Their chemical structures were confirmed by ultra performance liquid chromatogra-phy-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and ~1H, ~(13)C and 2D nuclear magnetic resonance (NMR). This represents the first report on direct separation of CAF and 24-mCAF from RBO by HPCCC.%Institute of Food and Nutraceutical Science, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China;Institute of Food and Nutraceutical Science, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China;Institute of Food and Nutraceutical Science, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China;Department of Food Science and Technology, University of Georgia, Athens, GA 30602-2610, USA;Institute of Food and Nutraceutical Science, Key Lab of Urban Agriculture (South), School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China,Department of Nutrition and Food Science, University of Maryland, 0112 Skinner Building, College Park. MD 20742. USA;