Hexafluoroisopropanol-induced salt-free catanionic surfactant coacervate extraction method for determination of fluoroquinolones in milk samples

摘要

Graphical abstractDisplay OmittedHighlights•Coacervation and phase separation in salt-free catanionic surfactant system.•HFIP-induced salt-free system is superior to salt-containing one in coacervation.•HFIP-induced salt-free coacervate extraction (salt-free CAE) is simple and rapid.•Salt-free CAE gave much higher enrichment factor for FQs than salt-containing one.•Salt-free CAE with HPLC-UV was used for detection of FQs in 13 kinds of milk.AbstractCoacervation and phase separation were first reported in salt-free catanionic surfactant aqueous systems based on lauric acid (LA) and dodecyltrimethylammonium hydroxide (DTAOH), using hexafluoroisopropanol (HFIP) as a coacervate-inducing agent. The liquid–liquid two-phase separation occurs over a wide of LA/DTAOH molar ratios (78:22–0:100mol/mol) and total surfactant concentrations (5–200mmolL−1) upon adding a small amount of HFIP (<10%, v/v). HFIP-induced salt-free LA/DTAOH catanionic surfactant system has much wider two-phase region than HFIP-induced salt-containing sodium laurate/dodecyltrimethylammonium bromide system. A HFIP-induced LA/DTAOH coacervate extraction method was established and coupled to high-performance liquid chromatography-ultraviolet detector (HPLC-UV) for determination of fluoroquinolones (rufloxacin, ciprofloxacin, danofloxacin, enrofloxacin) in milk. Detection limits are from 0.3ngmL−1to 1.4ngmL−1. Intra- and inter-day precisions (n=6) are in range of 4.5–8.3% and 5.8–10.7%, respectively. Recoveries are from 87.8% to 109.0%. The method, HFIP-induced salt-free coacervate extraction with HPLC-UV, is suitable for detecting trace fluoroquinolones in milk.