Determination of genome size of Haliotis discus hannai and H. diversicolor aquatilis (Haliotidae) and phylogenetic examination of this family

摘要

Genome size (C value) is an important index for phylogenetic studies. Haliotidae (abalones) includes many species widely distributed throughout the world’s oceans, which makes this family interesting for phylogenetic studies. To examine Haliotidae phylogeny, we determined the C value and adenine and thymine base pair content (AT %) of Haliotis discus hannai and H. diversicolor aquatilis by using flow cytometry. The C values of H. discus hannai and H. diversicolor aquatilis were 1.84 and 1.45 pg, with AT % of 62.3 and 66.3 %, respectively. These data represent the first report of abalones classified as Pacific Northwest (H. discus hannai) and Indo-Pacific (H. diversicolor aquatilis) groups, and provides new validation for previous theories related to Haliotidae phylogeny.