Optimum Dose of LH-RH Analogue Fertirelin Acetate for the Induction of Superovulation in Mice

摘要

The optimum dose for establishing superovulation in mice of Fertirelin Acetate (FA), an LH-RH analogue, was examined. Mice were subcutaneously injected with 5 IU of hCG at 17:00 (Day 0), and with various doses of FA (0.001 to 1.0 μg) five times at 4h intervals on and after 22:00 on Day 0. To induce ovulation, 5 IU of hCG was again injected subcutaneously at 17:00 on Day 2. In the groups administered with doses ranging from 0.01 to 0.5 μg of FA, the number of ovulated eggs was significantly (p<0.05) larger than in the control group (12.9 ± 5.9). The greatest number of ovulated eggs (22.6 + 7.3) was obtained in the group administered with 0.025 μg of FA. The results indicate that the effective dose of LH-RH analogue, FA, is between 0.1 and 0.5 μg for superovulation induction in mice.