The microalgae, Chlorella sp., were cultivated in various culture modes to assess biomass and lipid productivity in this study. In the batch mode, the biomass concentrations and lipid content of Chlorella sp. cultivated in a medium containing 0.025-0.200 g L~(-1) urea were 0.464-2.027 g L~(-1) and 0.661-0.326 g g~(-1), respectively. The maximum lipid productivity of 0.124 g d ~(-1) L~(-1) occurred in a medium containing 0.100 g L~(-1) urea. In the fed-batch cultivation, the highest lipid content was obtained by feeding 0.025 g L~(-1) of urea during the stationary phase, but the lipid productivity was not significantly increased. However, a semi-continuous process was carried out by harvesting the culture and renewing urea at 0.025 g L~(-1) each time when the cultivation achieved the early stationary phase. The maximum lipid productivity of 0.139 g d~(-1) L~(-1) in the semi-continuous culture was highest in comparison with those in the batch and fed-batch cultivations.
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机译：在本研究中，以各种培养模式培养微藻小球藻，以评估生物量和脂质生产率。在分批模式下，小球藻的生物量浓度和脂质含量。在含有0.025-0.200 g L〜（-1）尿素的培养基中培养的分别为0.464-2.027 g L〜（-1）和0.661-0.326 g g〜（-1）。在含0.100 g L〜（-1）尿素的培养基中，最大脂质生产率为0.124 g d〜（-1）L〜（-1）。在分批补料培养中，在固定期补饲0.025 g L〜（-1）的尿素可获得最高的脂质含量，但脂质生产率没有明显提高。但是，每当培养达到早期固定相时，通过收获培养物并更新尿素0.025 g L〜（-1）来进行半连续过程。与分批和分批分批培养相比，半连续培养的最大脂质产量为0.139 g d〜（-1）L〜（-1）。
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