Interactive effects on the EROD-inducing potency of polyhalogenated aromatic hydrocarbons in the chicken embryo hepatocyte assay

摘要

The chicken embryo hepatocyte-7-ethoxyresorufin O-deethylase (EROD) assay is used as a method to measure the amount of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (2, 3, 7, 8-TCDD) equivalents (TEQs) in environmental samples. A common feature of EROD-induction assays in vertebrates is that they generate biphasic dose-response relationships that show dose-related increases of the EROD induction to a maximum activity, followed by a dose-related decrease at higher concentration. In general, the maximum achievable enzyme activity decreases with increasing median effective concentration (EC50).